REPRODIVAC - Next-generation vaccines and diagnostics to prevent livestock reproductive diseases of worldwide impact
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Peripheral Blood Mononuclear Cells (PBMCs) Day
At the University of Lleida, Spain
Three of the Spanish REPRODIVAC teams celebrated yesterday the “PBMC´s Day”: Researchers and technicians from CITA and University of Navarra (UoN) visited the laboratories of the University of Lleida (UoL) with the aim of sharing and standardizing clinical and laboratory protocols to be used in their respective assays with pig peripheral blood mononuclear cells (PBMCs) and immune cells (PAMs) in the REPRODIVAC project. We compared the performance of different materials and protocols to obtain PBMCs from pig and determined the most useful ones for each situation (e.g. for sampling in vivo at commercial farms or for preparing big amounts of PBMC at the necropsy room in experiemtnal conditions). This session was very useful and inspiring for all the participants. The standardization of protocols will allow us to obtain comparable results within the framework of the REPRODIVAC project.




Paula Curto (UoL), Lorenzo Fraile (UoL), Maite Loperena (UoN), Pilar M. Muñoz (CITA), Raquel Conde (UoN), Miriam Salvador (UoN), Nerea López (UoN), Sara Andrés (CITA) and Ana Stoian (UoL).